Free PDF Protein Analysis and Purification: Benchtop Techniques, by Ian M. Rosenberg
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Protein Analysis and Purification: Benchtop Techniques, by Ian M. Rosenberg
Free PDF Protein Analysis and Purification: Benchtop Techniques, by Ian M. Rosenberg
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How one goes about analyzing proteins is a constantly evolving ?eld that is no longer solely the domain of the protein biochemist. Inves- gators from diverse disciplines ?nd themselves with the unanticipated task of identifying and analyzing a protein and studying its physical properties and biochemical interactions. In most cases, the ultimate goal remains understanding the role(s) that the target protein is playing in cellular physiology. It was my intention that this manual would make the initial steps in the discovery process less time consuming and less intimidating. This book is not meant to be read from cover to cover. The expanded Table of Contents and the index should help locate what you are seeking. My aim was to provide practically oriented information that will assist the experimentalist in benchtop problem solving. The appendices are ?lled with diverse information gleaned from catalogs, handbooks, and manuals that are presented in a distilled fashion designed to save trips to the library and calls to technical service representatives. The user is encouraged to expand on the tables and charts to ?t individual experimental situations. This second edition pays homage to the computer explosion and the various genome projects that have revolutionized how benchtop scienti?c research is performed. Bioinformatics and In silico science are here to stay. However, the second edition still includes recipes for preparing buffers and methods for lysing cells.
- Sales Rank: #6055661 in Books
- Published on: 2004-10-21
- Original language: English
- Number of items: 1
- Dimensions: 10.00" h x 1.19" w x 7.01" l, 2.47 pounds
- Binding: Hardcover
- 520 pages
From the Back Cover
New technologies and the manner in which scientific research is conducted have been fully incorporated into this thoroughly revised second edition of PROTEIN ANALYSIS AND PURIFICATION. Among the many topics introduced to this new edition are the use of robotics in the lab, the impact of the codification of the human genome, functional proteomics, mass spectrometry and the expanding use of bioinformatics as a tool to improve efficiency and productivity. The author has also included an annotated appendix of the educational materials and technical databases available online to enhance the researcher's benchside access to the latest resources. With solid explanations of the science asking the "why" and literally dozens of step-by-step protocols providing the "how", this new edition is a complete resource for the benchside.
Most helpful customer reviews
14 of 14 people found the following review helpful.
excellent and clearly presented
By A Customer
This is a model for all protocol lab manuals. It is clear, precise, and well done. Incorporates the experiences of the authors, which are used to perfect the assays and protocols and minimize insufficient results and data. Troubleshooting tips are useful and original. Truly a necessary benchtop reference for the serious researcher.
2 of 2 people found the following review helpful.
Practical Book
By Ruben Agrelo
Practical book with very easy to follow and concise protocols about protein purification
If you have to purify and analyze proteins but you re not an expert this is the book for you.
2 of 3 people found the following review helpful.
Very useful reference
By Armando-Malwani
Very useful reference for any biochemistry lab. Less bulkier than cold spring harbor manuals and certainly packed with a lot of useful information. This book would be useful for the professional as well as the novice. Detailed protocols included and updated from the previous edition. The section of post translational modifications was the most useful for my purposes. This book can take you from cells to purified protein under most circumstances with the minimum of repetition. I recommend this book particularly for graduate students in biochemistry who deal with protein purification as well as technicians.
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